Protein Purification Reagents and Kits
Biolegend APC anti-mouse Ly-6A/E Sca-1 R
APC anti-mouse Ly-6A/E (Sca-1) Recombinant [QA17A36]; Isotype: Rat IgG1, κ; Reactivity: Mouse; Apps: FC; Size: 100 μg
STEMCELL Technologies EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit, For processing: 1 x 10^9 cells
This kit isolates human CD4+CD127lowCD25+ regulatory T cells (Tregs) from fresh or previously frozen peripheral blood mononuclear cells (PBMCs) or leukapheresis samples. First, CD25+ cells are isolated by column-free immunomagnetic positive selection using EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CD25+ cells, and unwanted non-Tregs are targeted for depletion. The final isolated fraction contains highly purified CD4+CD127lowCD25+ cells that express high levels of FOXP3 and are immediately ready for downstream applications. An optional protocol allows for the isolation of CD4+CD25- responder T cells in parallel for use in functional studies. Following cell isolation with this EasySep™ kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands.
IBA LifeSciences 100 mM Biotin stock solution; 1 ml
Biotin stock solution for preparing a dissociation buffer when using the Strep-tag technology for cells (Fab-TACS/Nano-TACS, CD3/CD28 Streptamer, MHC I Streptamer). Biotin addition causes the rapid dissociation of isolation reagents from the cell surface. Thus, fully functional, highly pure, and non-induced cells are received.
STEMCELL Technologies EasySep™ Human Pan-Extracellular Vesicle Positive Selection Kit, For processing: 20 mL biofluid processed
The EasySep™ Human Pan-Extracellular Vesicle Positive Selection Kit isolates highly purified human extracellular vesicles from plasma/serum and cell culture conditioned medium by immunomagnetic positive selection. Extracellular vesicles are labeled with Tetrameric Antibody Complexes recognizing CD9, CD81, and CD63, and magnetic particles. Labeled extracellular vesicles are separated without columns using an EasySep™ magnet. Unwanted biofluid components are simply poured off, while desired extracellular vesicles remain in the tube. Isolated extracellular vesicles are available for downstream applications such as DNA/RNA extraction, Western blot, or mass spectometry.
Abcam Cell Cytotoxicity Assay Kit (Colorimetric)
Cell Cytotoxicity Assay Kit (Colorimetric)
The product is subject to the following: Abcam Restricted Use Statement
IPHASE PHARMA SERVICES LLC Human CD3+T Cells Positive Selection Kit,10 test
Immunocyte Positive Sorting Kit adopts the method of immunomagnetic bead positive sorting, which uses specific biotinylated antibodies to bind to the immunocytes in human PBMC (peripheral blood mononuclear cells) or human CBMC (umbilical cord blood mononuclear cells), and then incubates with the magnetic particles to make the target cells stay in the magnetic field under the action of the external magnetic field, so as to achieve the goal of immunocyte cells to be separated. The kits contains the Biotin-Antibodies,SA Nanobeads and Isolation Buffer.
STEMCELL Technologies RoboSep™ HLA Chimerism BC CD56 Positive Selection Kit, For processing: 30 mL buffy coat processed
The EasySep™ HLA Chimerism Buffy Coat CD56 Positive Selection Kit II is designed to isolate CD56+ cells from freshly prepared buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD56 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD56 antigen is expressed on NK cells and NKT cells.This product replaces the EasySep™ Human Buffy Coat CD56 Positive Selection Kit (Catalog #18085) and EasySep™ HLA Buffy Coat CD56 Positive Selection Kit (Catalog #18085HLA), for even faster cell isolations.
Biolegend FITC anti-human CD45RA
FITC anti-human CD45RA [S23030B] Isotype Mouse IgG1 Reactivity Human Apps FC Size 25 tests
LifeSensors Tandem Ubiquitin Binding Entities (TUBE1) Agarose 1mL
Based on protein domains known to possess an affinity for ubiquitin, Tandem Ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitinated proteins. TUBEs display up to a 1000-fold increase in affinity for polyubiquitin moieties over the single ubiquitin binding associated domain (UBA). In addition, TUBEs protect polyubiquitinated proteins from deubiquitination and proteasomal degradation, allowing for detection at relatively low abundance. These properties effectively allow TUBEs to “capture” protein in its polyubiqutinated state. TUBEs can be used to isolate, enrich, and identify ubiquitinated proteins from cell and tissue extracts. Agarose coupled TUBEs allow for efficient recovery of polyubiquitinated proteins in a single step. Polyubiquitinated protein can easily be separated from TUBEs for proteomic studies or identification by Western blotting. This product is protected by one or more US or Foreign patents.
Biochain Institute Inc Membrane Protein - Human Adult Normal Tissue: Brain: Temporal Lobe, 0.1 mg/PK
Tissue membrane protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis. The protein is stored in a buffer with protease inhibitor cocktail.
Genscript Corporation MonoRab Anti-DYKDDDDK Magnet
GenScript MonoRab sup TM /sup Anti-DYKDDDDK Magnetic Beads are super paramagnetic beads with diameter of 70 m covalently coated with a rabbit monoclonal anti-DYKDDDDK antibody The beads are supplied as 50% slurry in TBS with 50% glycerol and 0 02% sodium azide The MonoRab sup TM /sup Anti-DYKDDDDK Magnetic Beads have a binding capacity of 1 5 mg DYKDDDDK-tagged protein (Size 50KDa) per 1 ml settled beads The beads can be re-used for at least 5 times Based on our new rabbit monoclonal antibody technology (MonoRab sup TM /sup ) this beads offers high binding capacity high purity and withstand stringent washing steps especially with buffers containing high concentration of salt The beads are resistant to up to 2 M NaCl and to buffers with pH as low as 2 5
Immunology Consultants anti-DYKDDDDK (Flag) Antibody Chicken - Affinity Purified
This 0.1 mg format of the affinity‑purified anti‑DYKDDDDK (FLAG) antibody provides the same high specificity and strong performance as the full‑size vial, ideal for smaller‑scale assays or pilot studies. Produced in chicken, this antibody delivers excellent sensitivity and low background across ELISA, Western blotting, immunoprecipitation, and general FLAG‑tag detection workflows. Its affinity‑purified preparation ensures consistent, reproducible results when detecting FLAG‑tagged recombinant proteins in expression and purification experiments. A cost‑effective option for labs needing high‑quality FLAG detection in a smaller volume.
Hampton Research HR4-957/Mounted CryoLoop - 20 micron, 0.2 - 0.3 mm - 25 pack
HR4-957/Mounted CryoLoop - 20 micron, 0.2 - 0.3 mm - 25 pack. Please note items from this supplier are non-returnable.